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Overview Of Multiplex Immunohistochemistry/Immunofluorescence

Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high interobserver variability and the capacity to label only one marker per tissue section.

This overview describes the various highly multiplexed techniques that have been developed to circumvent these limitations and allow the simultaneous detection of multiple markers from a single tissue section and a comprehensive study of cell composition, cellular function, and cell-cell interactions. You can also get more information about multiplex immunohistochemistry via

Among these techniques, multiplex immunohistochemistry/immunofluorescence (mIHC/IF) has shown particular promise. mIHC/IF offers robust multiplex staining and standardized quantitative analysis for highly reproducible, efficient and cost-effective tissue examinations.

Image Source: Google

This technique has direct potential for translational research and clinical practice, especially in the era of cancer immunotherapy. Conventional immunohistochemistry (IHC) is widely used as a diagnostic method in the field of tissue pathology but has certain limitations.

The most important of these is that this technique allows only one marker to be tagged per network section. This results in lost opportunities to obtain important prognostic and diagnostic information from patient samples.

Immunohistochemical/immunofluorescent (mIHC/IF) multiplex technology, which allows simultaneous detection of multiple markers on a single tissue section, was introduced and adopted in response to the increasing demand for better techniques in research and clinics.

A number of highly multiplexed tissue imaging technologies have also been developed that allow extensive studies of cell composition, functional status, and cell-cell interactions, demonstrating increased diagnostic utility.